Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity

Cristine P Lopez; Derick Erl P Sumalapao; Nelson R Villarante

doi:10.5455/njppp.2017.7.0412011052017

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Original Research
Online Published: 19 May 2017

Natl J Physiol Pharm Pharmacol. 2017; 7(9): 972-976

doi: 10.5455/njppp.2017.7.0412011052017

Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante.

Cited By:17

Abstract
Background: There is an increasing incidence of liver failure and lack of effective drugs for liver diseases.

Aim and Objective: This study identified the phytochemicals present in aqueous and ethanolic Bixa orellana L. leaf extracts and investigated the hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. Specifically, the study compared serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and examined liver tissues histopathology.

Materials and Methods: Aqueous and ethanolic B. orellana L. leaf extracts were prepared and screened for phytochemical contents. An oral dose of 500 mg/kg body weight was identified from toxicity test and administered to albino mice. Microscopic examination of the liver tissues assessed the extent of hepatic injury. Serum AST and ALT levels were compared using one-way analysis of variance with Bonferroni post hoc analysis at 5% level of significance using Stata/SE V12.0 software.

Results: B. orellana L. leaves contained alkaloids, anthraquinones, sugars, and tannins. Aqueous and ethanolic leaf extracts of B. orellana L. did not show any toxicity up to 2000 mg/kg body weight oral dose in mice. Pre-treatment for 7 days before CCl4 administration significantly prevented elevation of serum AST and ALT levels with histopathologic findings showing a protective effect on the hepatocytes.

Conclusion: B. orellana L. leaves have potent hepatoprotective activity against oxidative damage.

Key words: Bixa orellana L.; Leaf Extract; Carbon Tetrachloride; Hepatotoxicity; Liver Enzymes; Oxidative Damage

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Pubmed Style

Lopez CP, Sumalapao DEP, Villarante NR. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. 2017; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017

Web Style

Lopez CP, Sumalapao DEP, Villarante NR. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. https://www.njppp.com/?mno=264257 [Access: November 01, 2025]. doi:10.5455/njppp.2017.7.0412011052017

AMA (American Medical Association) Style

Lopez CP, Sumalapao DEP, Villarante NR. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. 2017; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017

Vancouver/ICMJE Style

Lopez CP, Sumalapao DEP, Villarante NR. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. (2017), [cited November 01, 2025]; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017

Harvard Style

Lopez, C. P., Sumalapao, . D. E. P. & Villarante, . N. R. (2017) Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017

Turabian Style

Lopez, Cristine P, Derick Erl P Sumalapao, and Nelson R Villarante. 2017. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017

Chicago Style

Lopez, Cristine P, Derick Erl P Sumalapao, and Nelson R Villarante. "Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 972-976. doi:10.5455/njppp.2017.7.0412011052017

MLA (The Modern Language Association) Style

Lopez, Cristine P, Derick Erl P Sumalapao, and Nelson R Villarante. "Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity." National Journal of Physiology, Pharmacy and Pharmacology 7.9 (2017), 972-976. Print. doi:10.5455/njppp.2017.7.0412011052017

APA (American Psychological Association) Style

Lopez, C. P., Sumalapao, . D. E. P. & Villarante, . N. R. (2017) Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017

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