E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification

Ayandip Nandi, Suvendu Sengupta, Kausik Das, Manas Talukdar.

Abstract
Background: Diffuse large B-cell lymphoma (DLBCL) represents a heterogeneous group of lymphoid malignancies with distinct oncogenic events and clinical behavior that cannot be unraveled by morphology and immunophenotype alone. Simple biological segregation such as the Hans classifier helps to explain the heterogeneous responses to standard treatment and provides a rationale for the investigation of novel targeted therapies.

Aims and Objectives: In this study, we tried to estimate immunohistochemical (IHC) features of nodal and extranodal DLBCL using cell-of-origin classification (Hans Algorithm) with the markers CD10, Bcl-6, MUM1.

Materials and Methods: Blocks of the patients with a histological diagnosis of DLBCL over the past 3 years were retrieved and submitted for IHC analysis to classify into germinal center B cell type (GCB) and non-GCB type.

Results: Mean age for nodal DLBL was 48.19 ± 14.68 years, extranodal were 55.7 ± 13.22 years. Mean age for GCB were 56.6 ± 15.66 years whereas for non-GCB were 51.45 ± 12.85 years. Among nodal lymphomas cervical was the most common site and among extranodal lymphomas, intestinal lymphomas were commonest (including colorectal). Relative proportion of GCB among extranodal was 28.78%, whereas in nodal DLBCL it was 16.67%, relative risk of getting GCB type DLBCL was 1.72 times higher in extranodal compared to nodal DLBCL (P = 0.081). Total positivity of MUM1 was 17%, whereas for Bcl6 and CD10 it was 29% and 15% respectively. Ki67 was considerably higher in GCB type and for extranodal DLBCL in our study.

Conclusion: Proportion of extranodal GCB type DLBCL compared to nodal DLBCL is considerably higher in our study population, though it varies greatly among Asian and world data. Uniform meta-analysis and systematic review is necessary to stratify.

Key words: Diffuse Large B Cell Lymphoma; Immunohistochemistry; Non-Hodgkin; B-cell Lymphoma 6 Protein; Human


 
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How to Cite this Article
Pubmed Style

Nandi A, Sengupta S, Das K, Talukdar M. Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. Natl J Physiol Pharm Pharmacol. 2022; 12(9): 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022


Web Style

Nandi A, Sengupta S, Das K, Talukdar M. Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. https://www.njppp.com/?mno=7914 [Access: September 01, 2022]. doi:10.5455/njppp.2022.12.01042202222012022


AMA (American Medical Association) Style

Nandi A, Sengupta S, Das K, Talukdar M. Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. Natl J Physiol Pharm Pharmacol. 2022; 12(9): 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022



Vancouver/ICMJE Style

Nandi A, Sengupta S, Das K, Talukdar M. Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. Natl J Physiol Pharm Pharmacol. (2022), [cited September 01, 2022]; 12(9): 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022



Harvard Style

Nandi, A., Sengupta, . S., Das, . K. & Talukdar, . M. (2022) Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. Natl J Physiol Pharm Pharmacol, 12 (9), 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022



Turabian Style

Nandi, Ayandip, Suvendu Sengupta, Kausik Das, and Manas Talukdar. 2022. Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. National Journal of Physiology, Pharmacy and Pharmacology, 12 (9), 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022



Chicago Style

Nandi, Ayandip, Suvendu Sengupta, Kausik Das, and Manas Talukdar. "Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification." National Journal of Physiology, Pharmacy and Pharmacology 12 (2022), 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022



MLA (The Modern Language Association) Style

Nandi, Ayandip, Suvendu Sengupta, Kausik Das, and Manas Talukdar. "Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification." National Journal of Physiology, Pharmacy and Pharmacology 12.9 (2022), 1501-1505. Print. doi:10.5455/njppp.2022.12.01042202222012022



APA (American Psychological Association) Style

Nandi, A., Sengupta, . S., Das, . K. & Talukdar, . M. (2022) Comparative analysis of immunohistochemical features of nodal versus extranodal diffuse large B cell lymphoma with respect to cell-of-origin classification. National Journal of Physiology, Pharmacy and Pharmacology, 12 (9), 1501-1505. doi:10.5455/njppp.2022.12.01042202222012022