E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


A novel method of peripheral blood smear preparation

Prashant D Khuje, Bharti U Sable.

Abstract
Background: Peripheral blood smear (PBS) preparation using conventional wedge slide technique and staining with Leishman stain is still prescribed in medical undergraduate syllabus in physiology.

Aims and Objectives: The present study was undertaken to overcome some technical errors such as cross-striations and Rouleaux formation encountered during conventional method.

Materials and Methods: 20 PBS were prepared by present method of diluting whole blood with 0.9% NaCl using white blood cell (WBC) pipette, preparing smear using smooth edged spreader inclined at an angle of 60° followed by staining with Leishman’s stain.

Results: Statistical analysis by Fisher’s exact test revealed that 19 PBS prepared by present method showed uniformly thin film without cross-striations against 16 in controls which was statistically insignificant (P = 0.3416) (P > 0.05). 18 PBS prepared by modified method showed evenly distributed red blood cells and scattered WBCs without Rouleaux formation against 11 in controls which was statistically significant (P = 0.031) (P < 0.05).

Conclusion: Present method might be considered as simple and easy method of preparation of fairly ideal PBS as it overcame some errors encountered during conventional method such as cross-striations and Rouleaux formation.

Key words: Manual Method; Peripheral Blood Smear; Novel Method


 
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Pubmed Style

Khuje PD, Sable BU. A novel method of peripheral blood smear preparation. Natl J Physiol Pharm Pharmacol. 2021; 11(8): 821-823. doi:10.5455/njppp.2021.11.02045202107032021


Web Style

Khuje PD, Sable BU. A novel method of peripheral blood smear preparation. http://www.njppp.com/?mno=51462 [Access: October 02, 2021]. doi:10.5455/njppp.2021.11.02045202107032021


AMA (American Medical Association) Style

Khuje PD, Sable BU. A novel method of peripheral blood smear preparation. Natl J Physiol Pharm Pharmacol. 2021; 11(8): 821-823. doi:10.5455/njppp.2021.11.02045202107032021



Vancouver/ICMJE Style

Khuje PD, Sable BU. A novel method of peripheral blood smear preparation. Natl J Physiol Pharm Pharmacol. (2021), [cited October 02, 2021]; 11(8): 821-823. doi:10.5455/njppp.2021.11.02045202107032021



Harvard Style

Khuje, P. D. & Sable, . B. U. (2021) A novel method of peripheral blood smear preparation. Natl J Physiol Pharm Pharmacol, 11 (8), 821-823. doi:10.5455/njppp.2021.11.02045202107032021



Turabian Style

Khuje, Prashant D, and Bharti U Sable. 2021. A novel method of peripheral blood smear preparation. National Journal of Physiology, Pharmacy and Pharmacology, 11 (8), 821-823. doi:10.5455/njppp.2021.11.02045202107032021



Chicago Style

Khuje, Prashant D, and Bharti U Sable. "A novel method of peripheral blood smear preparation." National Journal of Physiology, Pharmacy and Pharmacology 11 (2021), 821-823. doi:10.5455/njppp.2021.11.02045202107032021



MLA (The Modern Language Association) Style

Khuje, Prashant D, and Bharti U Sable. "A novel method of peripheral blood smear preparation." National Journal of Physiology, Pharmacy and Pharmacology 11.8 (2021), 821-823. Print. doi:10.5455/njppp.2021.11.02045202107032021



APA (American Psychological Association) Style

Khuje, P. D. & Sable, . B. U. (2021) A novel method of peripheral blood smear preparation. National Journal of Physiology, Pharmacy and Pharmacology, 11 (8), 821-823. doi:10.5455/njppp.2021.11.02045202107032021