E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy.

Abstract
Background: Diabetic nephropathy (DN) is a progressive and irreversible renal disease. Experimental researches have established the role of oxidative stress as a central factor in pathogenesis, onset, and advancement of DN.

Aims and Objectives: To investigate the effect of the combined treatment with angiotensin-converting enzyme inhibitor, enalapril, and the specific vitamin D receptor activator paricalcitol, alone or in combination, using a diabetic rat model.

Materials and Methods: Fifty rats were divided into five groups of 10 rats each. Diabetes was induced by a single intraperitoneal injection of streptozotocin (50 mg/kg bw). Group 1 consisting of normal rats was served as control group. Group 2 was treated with vehicle (100 μl propylene glycol ip), group 3 was treated with enalapril (25 mg/l in drinking water), group 4 was treated with paricalcitol (0.8 μg/kg ip, 3 × weeks), and group 5 was treated with both drugs. The rats were treated for 3 months. Biochemical analysis was performed using an automatic biochemistry analyzer. Evaluation of oxidant/antioxidant balance and immunohistochemical localization of 3-nitrotyrosine (3-NT) in the kidney tissue was performed.

Results: Combined treatment with both drugs was associated with significantly lower blood glucose, malondialdehyde, nitric oxide, levels and significantly higher levels of the antioxidant parameters more than those observed for monotherapy. Co-treatment led to additional improvement with negligible interstitial damage with no glomerular or tubular injury detected and strongly decreased 3-NT expression induced by diabetes.

Conclusion: Co-treatment with both drugs exerts a synergistic protective effect against diabetic nephropathy by decreasing oxidative and nitrosative stress.

Key words: Enalapril; Paricalcitol; Oxidative and Nitrosative Stress; Diabetic Nephropathy; Rats


 
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How to Cite this Article
Pubmed Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. Natl J Physiol Pharm Pharmacol. 2015; 5(3): 222-231. doi:10.5455/njppp.2015.5.1401201522


Web Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. https://www.njppp.com/?mno=177563 [Access: February 03, 2024]. doi:10.5455/njppp.2015.5.1401201522


AMA (American Medical Association) Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. Natl J Physiol Pharm Pharmacol. 2015; 5(3): 222-231. doi:10.5455/njppp.2015.5.1401201522



Vancouver/ICMJE Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. Natl J Physiol Pharm Pharmacol. (2015), [cited February 03, 2024]; 5(3): 222-231. doi:10.5455/njppp.2015.5.1401201522



Harvard Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy (2015) Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. Natl J Physiol Pharm Pharmacol, 5 (3), 222-231. doi:10.5455/njppp.2015.5.1401201522



Turabian Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. 2015. Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 5 (3), 222-231. doi:10.5455/njppp.2015.5.1401201522



Chicago Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. "Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 5 (2015), 222-231. doi:10.5455/njppp.2015.5.1401201522



MLA (The Modern Language Association) Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy. "Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 5.3 (2015), 222-231. Print. doi:10.5455/njppp.2015.5.1401201522



APA (American Psychological Association) Style

Tarek Mohamed Ali, Basem Hassan El Esawy, Elsayed A Elmorsy (2015) Effect of combining an angiotensin-converting enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 5 (3), 222-231. doi:10.5455/njppp.2015.5.1401201522