Service by

Home Journal Issues Guide for Authors Editorial Board About Aims & Scope Editorial Contact


Original Research Open Access


Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant

Sulistiyaningsih Sulistiyaningsih, Nabila Mudin S, Imam Adi Wicaksono, Arif Budiman.

Abstract
Background: Nosocomial infection is a hospital-acquired infection that can cause various severe diseases. The main bacteria that causes nosocomial infection is Pseudomonas aeruginosa multiresistant (PAMR). The Rambutan leaf (Nephelium lappaceum) is known to have antibacterial activity because it is suspected to have the content of flavonoids, polyphenols, and tannins.

Aims and Objectives: This research was conducted to determine the antibacterial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of the extract and the most active fraction.

Materials and Methods: The research was started by extraction and then fractinationed by liquid-liquid extraction to obtain ethyl acetate, water, and n-hexane fraction. After that, antibacterial activity was carried out by the agar diffusion method, and the value of MIC and MBC was determined by the microdilution method.

Results: The result showed that ethanol extract, ethyl acetate fraction, and water fraction of the Rambutan leaf had antibacterial activity against PAMR, of which the ethyl acetate fraction was the most active fraction. The value of MIC and MBC of the extract and ethyl acetate fraction was in a concentration 2.5% w/v and 5% w/v against PAMR.

Conclusions: The antibacterial activity of this extract and fractions against PAMR was probably derived from flavonoids, polyphenols, saponins, and tannins.

Key words: Nosocomial Infection; Nephelium lappaceum; Antibacterial; Pseudomonas aeruginosa Multiresistant


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Sulistiyaningsih Sulistiyaningsih
Articles by Nabila Mudin S
Articles by Imam Adi Wicaksono
Articles by Arif Budiman
on Google
on Google Scholar
Article Statistics
 Viewed: 211
Downloaded: 92
Cited: 0


How to Cite this Article
Pubmed Style

Sulistiyaningsih S, S NM, Wicaksono IA, Budiman A. Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. Natl J Physiol Pharm Pharmacol. 2018; 8(2): 257-261. doi:10.5455/njppp.2017.7.0935926102017


Web Style

Sulistiyaningsih S, S NM, Wicaksono IA, Budiman A. Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. http://www.njppp.com/?mno=278133 [Access: February 23, 2018]. doi:10.5455/njppp.2017.7.0935926102017


AMA (American Medical Association) Style

Sulistiyaningsih S, S NM, Wicaksono IA, Budiman A. Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. Natl J Physiol Pharm Pharmacol. 2018; 8(2): 257-261. doi:10.5455/njppp.2017.7.0935926102017



Vancouver/ICMJE Style

Sulistiyaningsih S, S NM, Wicaksono IA, Budiman A. Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. Natl J Physiol Pharm Pharmacol. (2018), [cited February 23, 2018]; 8(2): 257-261. doi:10.5455/njppp.2017.7.0935926102017



Harvard Style

Sulistiyaningsih, S., S, N. M., Wicaksono, I. A. & Budiman, A. (2018) Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. Natl J Physiol Pharm Pharmacol, 8 (2), 257-261. doi:10.5455/njppp.2017.7.0935926102017



Turabian Style

Sulistiyaningsih, Sulistiyaningsih, Nabila Mudin S, Imam Adi Wicaksono, and Arif Budiman. 2018. Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. National Journal of Physiology, Pharmacy and Pharmacology, 8 (2), 257-261. doi:10.5455/njppp.2017.7.0935926102017



Chicago Style

Sulistiyaningsih, Sulistiyaningsih, Nabila Mudin S, Imam Adi Wicaksono, and Arif Budiman. "Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 257-261. doi:10.5455/njppp.2017.7.0935926102017



MLA (The Modern Language Association) Style

Sulistiyaningsih, Sulistiyaningsih, Nabila Mudin S, Imam Adi Wicaksono, and Arif Budiman. "Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant." National Journal of Physiology, Pharmacy and Pharmacology 8.2 (2018), 257-261. Print. doi:10.5455/njppp.2017.7.0935926102017



APA (American Psychological Association) Style

Sulistiyaningsih, S., S, N. M., Wicaksono, I. A. & Budiman, A. (2018) Antibacterial activity of ethanol extract and fraction of Rambutan leaf (Nephelium lappaceum) against Pseudomonas aeruginosa multiresistant. National Journal of Physiology, Pharmacy and Pharmacology, 8 (2), 257-261. doi:10.5455/njppp.2017.7.0935926102017



AUTHOR LOGIN

REVIEWER LOGIN

Indexed In

 

National Journal of Physiology, Pharmacy and Pharmacology is Indexed in -

EMBASE

Index Copernicus  (ICV 2016: 109)

Index Medicus for SEAR

CAB abstract/Global Health

CINAHL

Hinari

Google Scholar

ScopeMed

Ulrich’s International Periodical Directory

Indian Science Abstracts

Open J- Gate

Geneva Foundation for Medical Education and Research

NewJour

PSOAR

InformIndia

EBSCO Publishing Electronic Database

Genamics JournalSeek

ProQuest

Index Scholar

SafetyLit

Chemical Abstracts Service (CAS)

Gale's Health Reference Center

Gale Info Trac

Gale Academic Onefile

Ind Medica

Scirus

Scientific Publication Index

Scientific Resources Database

Recent Science Index

Scholarly Journals Index

Directory of Academic Resources

Elite Scientific Journals Archives

Current Index to Scholarly Journals

Digital Journals Database

Academic Papers Database

Contemporary Research Index

Ayush Web Portal

CiteFactor

 

 

 

 

 

 

Journal Membership

World Association of Medical Editors (WAME), Dallas, Texas, USA

 

Journal Listed in

ICMJE- International Committee of Medical Journal Editors

HIFA 2015 – Healthcare Information for All by 2015, Charlbury, Oxfordshire, UK 

 

 


The articles in this Journal are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.