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A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.

Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee.

Cited by (7)

Abstract
Background: Gentamicin is very useful aminoglycoside in treatment of gram negative organisms but nephrotoxicity has been a major limiting factor. Various renal pathologies have shown correlation with generation of oxidative stress. So, it was hypothesized that there may be involvement of oxidative stress in gentamicin nephrotoxicity.

Aims & Objective: To investigate the role of oxidative stress in gentamicin-induced nephrotoxicity and its prevention by using antioxidant vitamin C in Wistar albino rats. Another objective was to localize the site of kidney damage produced by gentamicin.

Materials and Methods: Male Wistar albino rats were administered gentamicin at the dose of 70 mg/kg/day i.m; either alone or in combination with vitamin C. Parameters of oxidative stress like glutathione peroxidase (GPx), glutathione reductase (GR) and total antioxidant status (TAS) were analysed. Renal damage was measured by blood urea and serum creatinine. Histopathological examination of rat kidney was
done. The data was subjected to one way ANOVA with post hoc test of significance and the values of P < 0.05 were considered statistically significant.

Results: Treatment with gentamicin caused significant reduction of TAS, GPx and GR activity, while blood urea and serum creatinine level were raised significantly as compared to control. Renal damage was confirmed with histopathological studies. The primary site of damage was identified as tubules especially proximal convoluted tubules. Administration of vitamin C alone raised TAS level significantly. Co-administration of vitamin C along with gentamicin led to significant restoration of GPx, GR, TAS, blood urea and serum creatinine level.

Conclusion: Renal damage due to gentamicin is associated with oxidative stress. The primary site of damage was renal proximal convoluted tubules. Co-administration of vitamin C along with gentamicin significantly prevented nephrotoxicity by virtue of its antioxidant effect.

Key words: Gentamicin, Nephrotoxicity, Vitamin C, Glutathione Peroxidase, Glutathione Reductase, Total Antioxidant status


 
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REFERENCES
1. Swan SK. Aminoglycoside nephrotoxicity. Semin Nephrol 1997;17:27-33.

[Pubmed]   

2. Sahnoun Z, Jamoussi K, Zeghal KM. Free radicals and antioxidants: human physiology, pathology and therapeutic aspects. Therapie 1997;52:251-70.

[
Pubmed]   

3. Hogg N. Free radicals in disease. Semin Reprod Endocrinol. 1998;16:41-8.

http://dx.doi.org/10.1055/s-2007-1016284

[
Pubmed]   

4. Knight JA. Free radicals: their history and current status in aging and disease. Ann Clin Lab Sci 1998;28:331-46.

[
Pubmed]   

5. Wayner DD, Burton GW, Ingold KU. The antioxidant efficiency of vitamin C is concentration-dependent. Biochem Biophys Acta 1986;884:119-23.

http://dx.doi.org/10.1016/0304-4165(86)90234-5


6. Padh H. Vitamin C: newer insights into its biochemical functions. Nutr Rev 1991;49:65-70.

http://dx.doi.org/10.1111/j.1753-4887.1991.tb07407.x

[
Pubmed]   

7. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of glutathion proxidase. J. Lab Med 1987;70:158-65.


8. Goldberg DM, Spooner RJ. In: Methods of enzymatic analysis. Bergmeyer HB editor. Vol. 3:258-6, 1992.


9. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science 1993;84:407412.

[
Pubmed]   

10. Walker PD, Barri Y, Shah SV. Oxidant mechanisms in gentamicin nephrotoxicity. Ren Fail 1999;21:433-42.

http://dx.doi.org/10.3109/08860229909085109

[
Pubmed]   

11. Walker PD, Shah SV. Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. J Clin Invest 1988;81:334-41.

http://dx.doi.org/10.1172/JCI113325

[
Pubmed]    [PMC Free Fulltext]   

12. Du XH, Yang CL. Mechanism of gentamicin nephrotoxicity in rats and the protective effect of zinc-induced metallothionein synthesis. Nephrol Dial Transplant 1994;9:135-40.

[
Pubmed]   

13. Sahnoun Z, Jamoussi K, Zeghal KM. Free radicals and antioxidants: human physiology, pathology and therapeutic aspects. Therapie 1997;52:251-70.

[
Pubmed]   

14. Hogg N. Free radicals in disease. Semin Reprod Endocrinol. 1998;16:41-8.

http://dx.doi.org/10.1055/s-2007-1016284

[
Pubmed]   

15. Knight JA. Free radicals: their history and current status in aging and disease. Ann Clin Lab Sci 1998;28:331-46.

[
Pubmed]   

16. Fujita T, Fujimoto Y. Formation and removal of active oxygen species and lipid peroxides in biological systems. Nippon Yakurigaku Zasshi 1992;99:381-9.

http://dx.doi.org/10.1254/fpj.99.381

[
Pubmed]   

17. Sandhya P, Varalakshmi P. Effect of lipoic acid administration on gentamicin-induced lipid peroxidation in rats. J Appl Toxicol 1997;17:405-8.

http://dx.doi.org/10.1002/(SICI)1099-1263(199711/12)17:6<405::AID-JAT459>3.0.CO;2-3


18. Soejima A, Ishizuka S, Suzuki M, Miyake N, Fukuoka K, Nagasawa T. Biochemical renal manifestations induced by consecutive administration of gentamicin in rats. Nephron 1998;80:331-9.

http://dx.doi.org/10.1159/000045194

[
Pubmed]   

19. Kavutcu M, Canbolat O, Ozturk S, Olcay E, Ulutepe S, Ekinci C et al. Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C. Nephron 1996;72:269-74.

http://dx.doi.org/10.1159/000188853

[
Pubmed]   

20. Coudert P, Leal F, Duroux E, Rubat C, Couquelet J. Effect on free radical processes of some ascorbic acid analogues. Biol Pharm Bull 1996;19:220-3.

http://dx.doi.org/10.1248/bpb.19.220

[
Pubmed]   

21. Fujita T, Fujimoto Y. Formation and removal of active oxygen species and lipid peroxides in biological systems. Nippon Yakurigaku Zasshi 1992;99:381-9.

http://dx.doi.org/10.1254/fpj.99.381

[
Pubmed]   

22. Padh H. Vitamin C: newer insights into its biochemical functions. Nutr Rev 1991;49:65-70.

http://dx.doi.org/10.1111/j.1753-4887.1991.tb07407.x

[
Pubmed]   

23. Wayner DD, Burton GW, Ingold KU. The antioxidant efficiency of vitamin C is concentration-dependent. Biochim Biophys Acta 1986;884:119-23.

http://dx.doi.org/10.1016/0304-4165(86)90234-5


24. Hong SK, Anestis DK, Ball JG, Valentovic MA, Brown PI, Rankin GO. 4-amino-2, 6-dichlorophenol (ADCP) induced nephrotoxicity in the Fischer 344 rat: protection by ascorbic acid, AT-125 and aminooxacetic acid. Fundam Appl Toxicol 1997;39:131-7.


25. Bradberry SM, Vale JA. Therapeutic review: is ascorbic acid of value in chromium poisoning and chromium dermatitis? J Toxicol Clin Toxicol 1999;37:195-200.

http://dx.doi.org/10.1081/CLT-100102419

[
Pubmed]   

26. Sha SH, Schacht J. Stimulation of free radical formation by aminoglycoside antibiotics. Hear Res 1999;128:112-8.

http://dx.doi.org/10.1016/S0378-5955(98)00200-7


27. Yang CL, Du XH, Han YX. Renal cortical mitochondria are the source of oxygen free radicals enhanced by gentamicin. Ren Fail 1995;17:21-6.

http://dx.doi.org/10.3109/08860229509036371

[
Pubmed]   

28. Walker PD, Shah SV. Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. J Clin Invest 1988;81:334-41.

http://dx.doi.org/10.1172/JCI113325

[
Pubmed]    [PMC Free Fulltext]   

29. Sha SH, Schacht J. Stimulation of free radical formation by aminoglycoside antibiotics. Hear Res 1999;128:112-8.

http://dx.doi.org/10.1016/S0378-5955(98)00200-7


30. Zima T, Tesar V, Stipek S, Nemecek K, Platenik J. The role of oxygen radicals in the pathogenesis of glomerulonephritis. Cas Lek Cesk 1995;134:716-9.

[
Pubmed]   

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How to Cite this Article
Pubmed Style

Acharya CR, Thakar HN, Vajpeyee SK. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20. doi:10.5455/njppp.2013.3.14-20


Web Style

Acharya CR, Thakar HN, Vajpeyee SK. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. http://www.njppp.com/?mno=22332 [Access: January 23, 2018]. doi:10.5455/njppp.2013.3.14-20


AMA (American Medical Association) Style

Acharya CR, Thakar HN, Vajpeyee SK. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20. doi:10.5455/njppp.2013.3.14-20



Vancouver/ICMJE Style

Acharya CR, Thakar HN, Vajpeyee SK. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. Natl J Physiol Pharm Pharmacol. (2013), [cited January 23, 2018]; 3(1): 14-20. doi:10.5455/njppp.2013.3.14-20



Harvard Style

Acharya, C. R., Thakar, H. N. & Vajpeyee, S. K. (2013) A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. Natl J Physiol Pharm Pharmacol, 3 (1), 14-20. doi:10.5455/njppp.2013.3.14-20



Turabian Style

Acharya, Chetankumar R., Hetal N. Thakar, and S. K. Vajpeyee. 2013. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. National Journal of Physiology, Pharmacy and Pharmacology, 3 (1), 14-20. doi:10.5455/njppp.2013.3.14-20



Chicago Style

Acharya, Chetankumar R., Hetal N. Thakar, and S. K. Vajpeyee. "A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.." National Journal of Physiology, Pharmacy and Pharmacology 3 (2013), 14-20. doi:10.5455/njppp.2013.3.14-20



MLA (The Modern Language Association) Style

Acharya, Chetankumar R., Hetal N. Thakar, and S. K. Vajpeyee. "A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.." National Journal of Physiology, Pharmacy and Pharmacology 3.1 (2013), 14-20. Print. doi:10.5455/njppp.2013.3.14-20



APA (American Psychological Association) Style

Acharya, C. R., Thakar, H. N. & Vajpeyee, S. K. (2013) A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.. National Journal of Physiology, Pharmacy and Pharmacology, 3 (1), 14-20. doi:10.5455/njppp.2013.3.14-20



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