E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research

Online Publishing Date:
11 / 05 / 2018

 


Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents

Rohit Dixit, Prabhakar Raosaheb Patil.


Abstract
Background: Although roots of neem plant have established pharmacological activities such as analgesic, anti-inflammatory, antimicrobial, and anticarcinogenic, there are contradictory results as far as anticonvulsant activity is concerned. Hence, the present study was undertaken to explore the anticonvulsant activity of neem roots.

Objectives: The objectives of this study were to study the anticonvulsant activity of alcoholic extract of neem root.

Materials and Methods: In maximal electroshock method (maximal electroshock seizure), a total of 30 albino rats of either sex weighing 200–250 g were used and divided into six groups. The control group received 2 ml of distilled water orally daily for 15 days. The standard group received phenobarbitone 20 mg/kg i.p. The test groups received orally neem leaf extract at the dose of 200, 400, and 800 mg/kg for 15 days. Convulsions were produced by 150 mA current for 0.2 s on day 1, 3, 5, and 10 using electroconvulsiometer. Change in duration of hind limb extension was measured. In the pentylenetetrazole (PTZ) method, PTZ at a dose of 60 mg/kg i.p was given to produce convulsion in all mice. A total of 30 mice were divided into five groups of six animals. The control group received orally 2 ml of distilled water daily for 15 days. The standard group received diazepam 4 mg/kg i.p. daily for 15 days. The test group received orally NLE at the dose of 200, 400, and 800 mg/kg for 15 days. The onset of clonic convulsions and the total number of clonic convulsions in 20 min was observed.

Results: The mean duration of hind limb extension in test group before the highest dose of neem extract (800 mg) administration was 8.7 ± 0.3 and after 15 days it was 8.8 ± 0.21. The mean onset of convulsions and mean number of convulsions in 20 min in the test group with highest dose of neem extract (800 mg) administration was 3.44 ± 0.24 and 42 ± 0.76, respectively, and there was no significant difference when compared to the control group.

Conclusion: The alcoholic extract of Azadirachta indica (neem) root is not having any anticonvulsant activity in rodents.

Key words: Neem; Rats; Mice; Experimental Pharmacology; Epilepsy; Plant Extract


 
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How to Cite this Article
Pubmed Style

Rohit Dixit, Prabhakar Raosaheb Patil. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. 2018; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018


Web Style

Rohit Dixit, Prabhakar Raosaheb Patil. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. https://www.njppp.com/?mno=295094 [Access: March 15, 2024]. doi:10.5455/njppp.2018.8.0310626042018


AMA (American Medical Association) Style

Rohit Dixit, Prabhakar Raosaheb Patil. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. 2018; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Vancouver/ICMJE Style

Rohit Dixit, Prabhakar Raosaheb Patil. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. (2018), [cited March 15, 2024]; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Harvard Style

Rohit Dixit, Prabhakar Raosaheb Patil (2018) Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Turabian Style

Rohit Dixit, Prabhakar Raosaheb Patil. 2018. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. National Journal of Physiology, Pharmacy and Pharmacology, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Chicago Style

Rohit Dixit, Prabhakar Raosaheb Patil. "Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



MLA (The Modern Language Association) Style

Rohit Dixit, Prabhakar Raosaheb Patil. "Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents." National Journal of Physiology, Pharmacy and Pharmacology 8.8 (2018), 1171-1174. Print. doi:10.5455/njppp.2018.8.0310626042018



APA (American Psychological Association) Style

Rohit Dixit, Prabhakar Raosaheb Patil (2018) Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. National Journal of Physiology, Pharmacy and Pharmacology, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018