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Original Research Open Access


Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense

Nina Salamah, Mustofa Ahda, Sidiq Bimantara, Rizka Hanar.

Abstract
Background: Ganoderma amboinense is rich in fibers, minerals, and vitamins. This antler-shaped mushroom contains various antioxidant compounds called polyphenols and flavonoids. Antioxidants can counteract or deactivate free radicals in plants and animals by turning them into less reactive compounds. The methods of compound extraction are numerous and with different effects on the concentration of active compounds. Hence, selecting a correct extraction method becomes necessary.

Aims and Objectives: The aim of this stuidy is to compare two extraction methods, namely, extraction by maceration and Soxhlet extraction, based on the total phenolic contents and the antioxidant activities of the ethanol extracts (96%) of G. amboinense.

Materials and Methods: The ethanol extract was obtained from maceration and Soxhlet extraction. The total phenolic content of 96% ethanol extract of G. amboinense was determined using ultraviolet-visible spectrophotometry with FolinľCiocalteu reagent, while the antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl method.

Results: Thin-layer chromatography produces ethanol extracts, from either maceration or Soxhlet extraction, that contain phenolic compounds. The total phenolic contents of the ethanol extracts from maceration and Soxhlet extraction are 26.72 ▒ 1.13 mg gallic acid equivalents (GAE)/g of extract and 23.27 ▒ 1.07 mg GAE/g of extract, respectively. The effective scavenging 50% values of gallic acid and ethanol extract are, respectively, 2.476 ╬╝g/ml and 197.6 ╬╝g/ml.

Conclusion: Maceration produces ethanol extract of G. amboinense with higher total phenolic content than Soxhlet extraction. The research findings indicate that the ethanol extract has in vitro antioxidant activity.

Key words: Phenolic; Antioxidant; Ganoderma; Mushroom


 
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How to Cite this Article
Pubmed Style

Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol. 2018; 8(1): 97-101. doi:10.5455/njppp.2018.8.0518614102017


Web Style

Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. http://www.njppp.com/?mno=267775 [Access: January 23, 2018]. doi:10.5455/njppp.2018.8.0518614102017


AMA (American Medical Association) Style

Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol. 2018; 8(1): 97-101. doi:10.5455/njppp.2018.8.0518614102017



Vancouver/ICMJE Style

Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol. (2018), [cited January 23, 2018]; 8(1): 97-101. doi:10.5455/njppp.2018.8.0518614102017



Harvard Style

Salamah, N., Ahda, M., Bimantara, S. & Hanar, R. (2018) Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol, 8 (1), 97-101. doi:10.5455/njppp.2018.8.0518614102017



Turabian Style

Salamah, Nina, Mustofa Ahda, Sidiq Bimantara, and Rizka Hanar. 2018. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. National Journal of Physiology, Pharmacy and Pharmacology, 8 (1), 97-101. doi:10.5455/njppp.2018.8.0518614102017



Chicago Style

Salamah, Nina, Mustofa Ahda, Sidiq Bimantara, and Rizka Hanar. "Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 97-101. doi:10.5455/njppp.2018.8.0518614102017



MLA (The Modern Language Association) Style

Salamah, Nina, Mustofa Ahda, Sidiq Bimantara, and Rizka Hanar. "Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense." National Journal of Physiology, Pharmacy and Pharmacology 8.1 (2018), 97-101. Print. doi:10.5455/njppp.2018.8.0518614102017



APA (American Psychological Association) Style

Salamah, N., Ahda, M., Bimantara, S. & Hanar, R. (2018) Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. National Journal of Physiology, Pharmacy and Pharmacology, 8 (1), 97-101. doi:10.5455/njppp.2018.8.0518614102017



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