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Original Research 


Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats

Kunjumon Dayana, Megaravalli R Manasa.

Abstract
Background: Chemopreventive compounds may play an important role in cancer prevention. However, many chemopreventive agents available currently are associated with toxicity. Hence, there is a need to screen for newer compounds with chemopreventive potential. Many medicinal plants can be developed as prospective chemopreventive candidates.

Aims and Objectives: The aim of the study was to assess the protective effect of ethanolic extract of calotropis procera root in 7,12-dimethylbenz[a]anthracene (DMBA) induced genotoxicity in Wistar rats by micronucleus assay.

Materials and Methods: Wistar rats were divided into 4 groups randomly. Group 1 received distilled water (control group). Group 2 was administered DMBA (30 mg/kg body weight [BW], single dose) intraperitoneally on the 5th day of the experiment. Group 3 rats were pretreated with C. procera root extract (500 mg/kg BW) orally for 5 days followed by DMBA injection intraperitoneally 2 h after C. procera root extract on the 5th day. Group 4 rats were given C. procera root alone orally for 5 days. The animals were sacrificed on the 6th day, and bone marrow was harvested for the micronucleus test. The percentage of micronuclei in polychromatic erythrocytes (%MnPCE) and PCE: NCE were determined.

Results: Group 2 rats exhibited significant (P < 0.0001) increase in the %MnPCEs compared to control group. Group 3 and 4 rats did not show a significant increase in %MnPCEs compared to control group. The PCE: NCE ratio is decreased in Group 2 rats compared to the control group, but it is not statistically significant. The PCE: NCE ratio does not differ significantly from the control group in Groups 3 and 4.

Conclusion: The ethanolic extract of C. procera root exhibits antigenotoxic activity in DMBA induced genotoxicity in Wistar rats.

Key words: Calotropis procera; 7,12-Dimethylbenz[a]anthracene; Antigenotoxic Effect; Wistar Rats


 
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Pubmed Style

Dayana K, Manasa MR. Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. Natl J Physiol Pharm Pharmacol. 2018; 8(12): 1617-1621. doi:10.5455/njppp.2018.8.0929013092018


Web Style

Dayana K, Manasa MR. Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. http://www.njppp.com/?mno=7966 [Access: December 13, 2018]. doi:10.5455/njppp.2018.8.0929013092018


AMA (American Medical Association) Style

Dayana K, Manasa MR. Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. Natl J Physiol Pharm Pharmacol. 2018; 8(12): 1617-1621. doi:10.5455/njppp.2018.8.0929013092018



Vancouver/ICMJE Style

Dayana K, Manasa MR. Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. Natl J Physiol Pharm Pharmacol. (2018), [cited December 13, 2018]; 8(12): 1617-1621. doi:10.5455/njppp.2018.8.0929013092018



Harvard Style

Dayana, K. & Manasa, . M. R. (2018) Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. Natl J Physiol Pharm Pharmacol, 8 (12), 1617-1621. doi:10.5455/njppp.2018.8.0929013092018



Turabian Style

Dayana, Kunjumon, and Megaravalli R Manasa. 2018. Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 8 (12), 1617-1621. doi:10.5455/njppp.2018.8.0929013092018



Chicago Style

Dayana, Kunjumon, and Megaravalli R Manasa. "Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 1617-1621. doi:10.5455/njppp.2018.8.0929013092018



MLA (The Modern Language Association) Style

Dayana, Kunjumon, and Megaravalli R Manasa. "Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 8.12 (2018), 1617-1621. Print. doi:10.5455/njppp.2018.8.0929013092018



APA (American Psychological Association) Style

Dayana, K. & Manasa, . M. R. (2018) Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 8 (12), 1617-1621. doi:10.5455/njppp.2018.8.0929013092018





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