E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats

Adam Gbankoto, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, Bonaventure Awede.

Abstract
Background: The use of natural products such as Moringa oleifera (MO) for the improvement of resistance artery relaxation is a potential strategy for the prevention and treatment of hypertension.

Aims and Objective: The present study was designed to evaluate the protective effect of MO hydro-ethanol extract on NG-nitro-L-arginine-methyl ester (L-NAME)-induced hypertension and the toxicity profile in Wistar rats and shrimp larvae.

Materials and Methods: An experimental hypertensive model was established by administration of L-NAME at 40 mg/kg body weight (BW) by oral gavages to male Wistar rats for 2 weeks and treated with hydro-ethanol extract of MO leaves and losartan. The control group received distilled water. L-NAME group was treated with L-NAME and distilled water. L-NAME-Losartan group was treated with L-NAME and losartan at 100 mg/kg BW. L-NAME-MO group was treated with L-NAME and the hydroethanol extract of MO leaves at 500 mg/kg BW. Blood pressure (BP) was measured by tail cuff method. In vitro toxicity study of the extract was performed on Artemia salina larvae using a biological test for the determination of shrimp lethality. A 14 days acute oral toxicity study was evaluated in female rats on a single dose of 2000 mg/kg BW of hydro-ethanol extract of MO.

Results: L-NAME treated rats developed significantly increased BP from 110.20 ± 4.46 mmHg to 159.60 ± 4.38 mmHg. Concurrent oral treatment with hydro-ethanol extract of MO leaves decreased the high BP from 159.60 ± 4.38 mmHg to 102.40 ± 5.07 mmHg. No harm was observed at the tested doses in vitro and in vivo toxicity studies.

Conclusion: These findings suggest that MO leaves may be potentially useful as a natural product against hypertension.

Key words: Moringa oleifera; Hypertension; NG-Nitro-L-Arginine-Methyl Ester; Wistar Rat; Toxicity


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Adam Gbankoto
Articles by Mariette Sindete
Articles by Marius Adjagba
Articles by Machioud Maxime Sangare
Articles by Eugene Selidji Attakpa
Articles by Bonaventure Awede
on Google
on Google Scholar
Article Statistics
 Viewed: 227
Downloaded: 37
Cited: 0


How to Cite this Article
Pubmed Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. 2019; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019


Web Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. http://www.njppp.com/?mno=68480 [Access: December 07, 2019]. doi:10.5455/njppp.2019.9.1034231102019


AMA (American Medical Association) Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. 2019; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Vancouver/ICMJE Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. (2019), [cited December 07, 2019]; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Harvard Style

Gbankoto, A., Sindete, . M., Adjagba, . M., Sangare, . M. M., Attakpa, . E. S. & Awede, . B. (2019) Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Turabian Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. 2019. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. National Journal of Physiology, Pharmacy and Pharmacology, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Chicago Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. "Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats." National Journal of Physiology, Pharmacy and Pharmacology 9 (2019), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



MLA (The Modern Language Association) Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. "Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats." National Journal of Physiology, Pharmacy and Pharmacology 9.12 (2019), 1257-1266. Print. doi:10.5455/njppp.2019.9.1034231102019



APA (American Psychological Association) Style

Gbankoto, A., Sindete, . M., Adjagba, . M., Sangare, . M. M., Attakpa, . E. S. & Awede, . B. (2019) Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. National Journal of Physiology, Pharmacy and Pharmacology, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019





Most Viewed Articles
  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Second MBBS medical student’s feedback on teaching methodology and evaluation methods in Pharmacology
    Nilesh Chavda, Preeti Yadav, Jay Karan, N. D. Kantharia
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 23-31
    » Abstract & References » doi: 10.5455/njppp.2011.1.23-31

  • Prophylaxis and Treatment for Leptospirosis: Where are the Evidences?
    Jaykaran Charan, Deepak Saxena, Summaiya Mulla
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 78-83
    » Abstract & References » doi: 10.5455/njppp.2012.2.78-83

  • How to write a thesis? [English]
    Swapnil s Agarwal, Preeti Yadav, Krishnadutt Chavali, Lavlesh Kumar
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 86-90
    » Abstract

  • Most Downloaded
  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • Evaluation of potential drug-drug interaction in indoor patients of pediatric department of tertiary care hospital
    Simran Patel, Mayur Chaudhari, Nrupal Patel
    Natl J Physiol Pharm Pharmacol. 2019; 9(10): 1012-1016
    » Abstract » doi: 10.5455/njppp.2019.9.0725209072019

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Comparison of bleeding time and clotting time between males and females
    Ritu Adhana, Rajanish Chaurasiya, Anjali Verma
    Natl J Physiol Pharm Pharmacol. 2018; 8(10): 1388-1390
    » Abstract » doi: 10.5455/ijmsph.2018.06201417062018

  • Effectiveness of “case-based learning” in physiology
    Amruta S Bennal, Manohar Y Pattar, Raju H Taklikar
    Natl J Physiol Pharm Pharmacol. 2016; 6(1): 65-67
    » Abstract & References » doi: 10.5455/njppp.2015.5.1810201583

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Most Cited Articles
  • Quercetin improved spatial memory dysfunctions in rat model of intracerebroventricular streptozotocin-induced sporadic Alzheimer’s disease
    Manouchehr Ashrafpour, Sahar Parsaei, Hamid Sepehri
    Natl J Physiol Pharm Pharmacol. 2015; 5(5): 411-415
    » Abstract & References » doi: 10.5455/njppp.2015.5.2308201563
    Cited : 11 times [Click to see citing articles]

  • A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.
    Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20
    » Abstract & References » doi: 10.5455/njppp.2013.3.14-20
    Cited : 10 times [Click to see citing articles]

  • Promoting active learning in respiratory physiology – Positive student perception and improved outcomes
    Richa Thaman, Sukhjinder Dhillon, Sanjeev Saggar, Meenakshi Gupta, Harkirat Kaur
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 27-34
    » Abstract & References » doi: 10.5455/njppp.2013.3.27000
    Cited : 10 times [Click to see citing articles]

  • Pattern of mobile phone usage and its effects on psychological health, sleep, and academic performance in students of a medical university
    Naveenta Gupta, Sonia Garg, Khushdeep Arora
    Natl J Physiol Pharm Pharmacol. 2016; 6(2): 132-139
    » Abstract & References » doi: 10.5455/njppp.2016.6.0311201599
    Cited : 8 times [Click to see citing articles]

  • Prevalence and risk factors for eating disorders in Indian adolescent females
    Amit A Upadhyah, Rajesh Misra, Deepak N Parchwani, Pankaj B Maheria
    Natl J Physiol Pharm Pharmacol. 2014; 4(2): 153-157
    » Abstract & References » doi: 10.5455/njppp.2014.4.041220131
    Cited : 6 times [Click to see citing articles]

  • Time domain measures of heart rate variability during acute mental stress in Type 2 diabetics- A case control study
    Anupama Deepak, Deepak A N, Shobha Nallulwar, Vitthal Khode
    Natl J Physiol Pharm Pharmacol. 2014; 4(1): 34-38
    » Abstract & References » doi: 10.5455/njppp.2014.4.080720131
    Cited : 6 times [Click to see citing articles]

  • Ratio of second to fourth digit as a predictor of performance in elite Indian volley ball players
    Kaninika Panda, Pralay Majumdar, Veena Umesh, HH Sudhakar
    Natl J Physiol Pharm Pharmacol. 2014; 4(2): 106-108
    » Abstract & References » doi: 10.5455/njppp.2014.4.011020132
    Cited : 6 times [Click to see citing articles]

  • A pilot study to evaluate the effect of HAART on gonadal dysfunction in male HIV patients
    Abhishek Pathak, Lalit Prashant Meena, Jaya Chakravarty, Madhukar Rai, Shyam Sundar
    Natl J Physiol Pharm Pharmacol. 2015; 5(1): 33-35
    » Abstract & References » doi: 10.5455/njppp.2015.5.120720142
    Cited : 6 times [Click to see citing articles]

  • Hesperidin protects against diethylnitrosamine-induced nephrotoxicity through modulation of oxidative stress and inflammation
    Rasha R Ahmed, Ayman M Mahmoud, Mohamed B Ashour, Amira M Kamel
    Natl J Physiol Pharm Pharmacol. 2015; 5(5): 391-397
    » Abstract & References » doi: 10.5455/njppp.2015.5.2907201567
    Cited : 6 times [Click to see citing articles]

  • Newer anti-platelet and anti-thrombotic drugs on the horizon
    Ajai Garg, Anjana Pandey, Pranab Oza, Mridul Chaturvedi
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 105-110
    » Abstract & References » doi: 10.5455/njppp.2013.3.090420131
    Cited : 6 times [Click to see citing articles]