E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats

Adam Gbankoto, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, Bonaventure Awede.

Abstract
Background: The use of natural products such as Moringa oleifera (MO) for the improvement of resistance artery relaxation is a potential strategy for the prevention and treatment of hypertension.

Aims and Objective: The present study was designed to evaluate the protective effect of MO hydro-ethanol extract on NG-nitro-L-arginine-methyl ester (L-NAME)-induced hypertension and the toxicity profile in Wistar rats and shrimp larvae.

Materials and Methods: An experimental hypertensive model was established by administration of L-NAME at 40 mg/kg body weight (BW) by oral gavages to male Wistar rats for 2 weeks and treated with hydro-ethanol extract of MO leaves and losartan. The control group received distilled water. L-NAME group was treated with L-NAME and distilled water. L-NAME-Losartan group was treated with L-NAME and losartan at 100 mg/kg BW. L-NAME-MO group was treated with L-NAME and the hydroethanol extract of MO leaves at 500 mg/kg BW. Blood pressure (BP) was measured by tail cuff method. In vitro toxicity study of the extract was performed on Artemia salina larvae using a biological test for the determination of shrimp lethality. A 14 days acute oral toxicity study was evaluated in female rats on a single dose of 2000 mg/kg BW of hydro-ethanol extract of MO.

Results: L-NAME treated rats developed significantly increased BP from 110.20 4.46 mmHg to 159.60 4.38 mmHg. Concurrent oral treatment with hydro-ethanol extract of MO leaves decreased the high BP from 159.60 4.38 mmHg to 102.40 5.07 mmHg. No harm was observed at the tested doses in vitro and in vivo toxicity studies.

Conclusion: These findings suggest that MO leaves may be potentially useful as a natural product against hypertension.

Key words: Moringa oleifera; Hypertension; NG-Nitro-L-Arginine-Methyl Ester; Wistar Rat; Toxicity


 
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How to Cite this Article
Pubmed Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. 2019; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019


Web Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. http://www.njppp.com/?mno=68480 [Access: October 01, 2020]. doi:10.5455/njppp.2019.9.1034231102019


AMA (American Medical Association) Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. 2019; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Vancouver/ICMJE Style

Gbankoto A, Sindete M, Adjagba M, Sangare MM, Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol. (2019), [cited October 01, 2020]; 9(12): 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Harvard Style

Gbankoto, A., Sindete, . M., Adjagba, . M., Sangare, . M. M., Attakpa, . E. S. & Awede, . B. (2019) Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. Natl J Physiol Pharm Pharmacol, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Turabian Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. 2019. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. National Journal of Physiology, Pharmacy and Pharmacology, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



Chicago Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. "Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats." National Journal of Physiology, Pharmacy and Pharmacology 9 (2019), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019



MLA (The Modern Language Association) Style

Gbankoto, Adam, Mariette Sindete, Marius Adjagba, Machioud Maxime Sangare, Eugene Selidji Attakpa, and Bonaventure Awede. "Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats." National Journal of Physiology, Pharmacy and Pharmacology 9.12 (2019), 1257-1266. Print. doi:10.5455/njppp.2019.9.1034231102019



APA (American Psychological Association) Style

Gbankoto, A., Sindete, . M., Adjagba, . M., Sangare, . M. M., Attakpa, . E. S. & Awede, . B. (2019) Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. National Journal of Physiology, Pharmacy and Pharmacology, 9 (12), 1257-1266. doi:10.5455/njppp.2019.9.1034231102019