Service by

Home Journal Issues Guide for Authors Editorial Board About Aims & Scope Editorial Contact


Original Research Open Access


Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Varshan Raj, Gopinath Pa, Ashish R Jain.

Abstract
Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps.

Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India.

Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of blaIMP gene by polymerase chain reaction analysis.

Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess blaIMP gene.

Conclusion: The early detection of MBL‑producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.

Key words: BlaIMP Gene; Pseudomonas aeruginosa; Polymerase Chain Reaction; Metallo-Beta-Lactamase


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Varshan Raj
Articles by Gopinath Pa
Articles by Ashish R Jain
on Google
on Google Scholar
Article Statistics
 Viewed: 56
Downloaded: 22
Cited: 0


How to Cite this Article
Pubmed Style

Raj V, Pa G, Jain AR. Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018


Web Style

Raj V, Pa G, Jain AR. Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. http://www.njppp.com/?mno=283969 [Access: February 23, 2018]. doi:10.5455/njppp.2018.8.114549012018


AMA (American Medical Association) Style

Raj V, Pa G, Jain AR. Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018



Vancouver/ICMJE Style

Raj V, Pa G, Jain AR. Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018



Harvard Style

Raj, V., Pa, G. & Jain, A. R. (2018) Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018



Turabian Style

Raj, Varshan, Gopinath Pa, and Ashish R Jain. 2018. Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018



Chicago Style

Raj, Varshan, Gopinath Pa, and Ashish R Jain. "Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa." doi:10.5455/njppp.2018.8.114549012018



MLA (The Modern Language Association) Style

Raj, Varshan, Gopinath Pa, and Ashish R Jain. "Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa." doi:10.5455/njppp.2018.8.114549012018



APA (American Psychological Association) Style

Raj, V., Pa, G. & Jain, A. R. (2018) Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa. doi:10.5455/njppp.2018.8.114549012018



AUTHOR LOGIN

REVIEWER LOGIN

Indexed In

 

National Journal of Physiology, Pharmacy and Pharmacology is Indexed in -

EMBASE

Index Copernicus  (ICV 2016: 109)

Index Medicus for SEAR

CAB abstract/Global Health

CINAHL

Hinari

Google Scholar

ScopeMed

Ulrich’s International Periodical Directory

Indian Science Abstracts

Open J- Gate

Geneva Foundation for Medical Education and Research

NewJour

PSOAR

InformIndia

EBSCO Publishing Electronic Database

Genamics JournalSeek

ProQuest

Index Scholar

SafetyLit

Chemical Abstracts Service (CAS)

Gale's Health Reference Center

Gale Info Trac

Gale Academic Onefile

Ind Medica

Scirus

Scientific Publication Index

Scientific Resources Database

Recent Science Index

Scholarly Journals Index

Directory of Academic Resources

Elite Scientific Journals Archives

Current Index to Scholarly Journals

Digital Journals Database

Academic Papers Database

Contemporary Research Index

Ayush Web Portal

CiteFactor

 

 

 

 

 

 

Journal Membership

World Association of Medical Editors (WAME), Dallas, Texas, USA

 

Journal Listed in

ICMJE- International Committee of Medical Journal Editors

HIFA 2015 – Healthcare Information for All by 2015, Charlbury, Oxfordshire, UK 

 

 


The articles in this Journal are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.