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Original Research Open Access


Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus

Salama Moustafa Abd-El-Hafez, Alaa Basyouni Ismael, Mohamed Mohamed Soliman, Essam Hassan Mohamed, Ibrahim Khalid Kafaween, Hany Hassan Mohamed.

Abstract
Background: The herbal medications are widely used in folk medicine for treatment of variable diseases and infection. Staphylococcus aureus (Sa) is a Gram-positive bacteria that cause severe organ affection, especially in the lung. Nigella sativa (NS) and gum arabic (GA) are probiotics with multi-beneficial effects for human health.

Aims and Objectives: The current study was outlined to examine the protective effect of NS and GA against SA induced serum alterations and changes in cytokines and antioxidants expressions.

Materials and Methods: A total of 42 male Wistar rats were allocated into six groups (6 each). Control group without any treatment; SA group in a dose of 2 109 colony forming units /mL per rat. Rats in 3-6 groups served as SA infected groups and received NS for Group 3 (150 mg/kg b.w. daily), GA plus SA for Group 4 (10% wt/vol. daily), and SA group received a mixture of NS plus GA for Group 5, and SA administered group received Lactobacillus for Group 6 as a positive control. NS and GA were administered 2 weeks before SA infection and continued for 1 week. Serum levels of cytokines and oxidative stress biomarkers were measured. Lung tissues were examined at molecular levels for mRNA expression of inflammatory cytokines and antioxidants.

Results: SA induced a significant decrease in serum levels of interleukin -6 (IL-6), IL-2, and tumor necrosis factor -alpha. NS induced significant decrease on examined cytokines levels in SA (P < 0.05). GA did not induce any additive changes in cytokines. When both NA and GA were administered together, NS induced additive inhibitory effect. SA injection induced significant increase in serum levels of catalase and glutathione reductase. NS induced additive significant increase on antioxidant levels in SA injected. GA did not induce any additive increase for examined antioxidant levels. NS decreased total bacterial count increased in SA group more significantly than the effect induced by NS.

Conclusion: NS has the potential to control the degree of inflammation at the protein and gene levels of examined cytokines (T-helper1/T-helper2) and antioxidants compared to the effect induced by GA.

Key words: Nigella Sativa; Gum Arabic; Lung; Staphylococcus aureus Infection; Protection


 
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How to Cite this Article
Pubmed Style

Abd-El-Hafez SM, Ismael AB, Soliman MM, Mohamed EH, Kafaween IK, Mohamed HH. Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. Natl J Physiol Pharm Pharmacol. 2017; 7(12): 1410-1416. doi:10.5455/njppp.2017.7.0936002102017


Web Style

Abd-El-Hafez SM, Ismael AB, Soliman MM, Mohamed EH, Kafaween IK, Mohamed HH. Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. http://www.njppp.com/?mno=278406 [Access: December 18, 2017]. doi:10.5455/njppp.2017.7.0936002102017


AMA (American Medical Association) Style

Abd-El-Hafez SM, Ismael AB, Soliman MM, Mohamed EH, Kafaween IK, Mohamed HH. Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. Natl J Physiol Pharm Pharmacol. 2017; 7(12): 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



Vancouver/ICMJE Style

Abd-El-Hafez SM, Ismael AB, Soliman MM, Mohamed EH, Kafaween IK, Mohamed HH. Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. Natl J Physiol Pharm Pharmacol. (2017), [cited December 18, 2017]; 7(12): 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



Harvard Style

Abd-El-Hafez, S. M., Ismael, A. B., Soliman, M. M., Mohamed, E. H., Kafaween, I. K. & Mohamed, H. H. (2017) Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. Natl J Physiol Pharm Pharmacol, 7 (12), 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



Turabian Style

Abd-El-Hafez, Salama Moustafa, Alaa Basyouni Ismael, Mohamed Mohamed Soliman, Essam Hassan Mohamed, Ibrahim Khalid Kafaween, and Hany Hassan Mohamed. 2017. Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. National Journal of Physiology, Pharmacy and Pharmacology, 7 (12), 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



Chicago Style

Abd-El-Hafez, Salama Moustafa, Alaa Basyouni Ismael, Mohamed Mohamed Soliman, Essam Hassan Mohamed, Ibrahim Khalid Kafaween, and Hany Hassan Mohamed. "Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



MLA (The Modern Language Association) Style

Abd-El-Hafez, Salama Moustafa, Alaa Basyouni Ismael, Mohamed Mohamed Soliman, Essam Hassan Mohamed, Ibrahim Khalid Kafaween, and Hany Hassan Mohamed. "Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus." National Journal of Physiology, Pharmacy and Pharmacology 7.12 (2017), 1410-1416. Print. doi:10.5455/njppp.2017.7.0936002102017



APA (American Psychological Association) Style

Abd-El-Hafez, S. M., Ismael, A. B., Soliman, M. M., Mohamed, E. H., Kafaween, I. K. & Mohamed, H. H. (2017) Effect of gum arabic and Nigella sativa on T-helper1 and T-helper2 immune response in Wistar rats infected with methicillin-resistant Staphylococcus aureus. National Journal of Physiology, Pharmacy and Pharmacology, 7 (12), 1410-1416. doi:10.5455/njppp.2017.7.0936002102017



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