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Original Research 


Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger

Shakiru Ademola Salami, Hussein Mofomosara Salahdeen, Gbolahan Olabisi Idowu, Babatunde Adekunle Murtala, Abdul Rasak Akinola Alada3.

Abstract
Background: Tridax procumbens leaf extract has been reported to show antihypertensive actions through its vasodilatory effects. Although several mechanisms of action have been reported, the sub-cellular mechanism of action of the extract is yet to be fully elucidated.

Aim and Objectives: In this study, we investigate the involvement of Na+ - K+ -pump and Na+- Ca2+ exchanger in the vasorelaxant activities of T. procumbens extract (TPE) in isolated rat superior mesenteric arteries.

Materials and Methods: Superior mesenteric artery isolated from healthy, young adult Wistar rats (250300 g) and pre-contracted with phenylephrine (PE) (10-7M) were treated with various concentrations of aqueous extract of TPE (0.59 mg/mL). The changes in arterial tension were recorded and interaction between TPE with the selective blocker of Na+ - K+ ATPase (ouabain) and putative Na+-Ca2+ exchanger inhibitor (NiCl2) was evaluated.

Results: The result indicated that TPE significantly inhibit the cumulative concentration response curves to PE (10-9 - 10-5 M) in a concentration-dependent manner. Pre-treatment with both ouabain and nickel chloride (NiCl2), abolished the vasorelaxant responses of TPE. Furthermore, TPE reduced the relaxant effect with decreased extracellular Na+ concentration. TPE-induced vasorelaxation was completely abolished in a Na+ -free solution, a condition that eliminates the influence of the forward mode of the exchanger.

Conclusion: The results provide direct hypothetical evidence that the activation of Na+ - K+ ATPase and stimulation of the forward mode of the Na+-Ca2+ exchanger contribute to the vasorelaxation induced by TPE in mesenteric artery.

Key words: Mesenteric Artery; Na+- K+- ATPase; Na+ - Ca2+ exchanger; Tridax procumbens


 
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How to Cite this Article
Pubmed Style

Salami SA, Salahdeen HM, Idowu GO, Murtala BA, Alada ARA. Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. Natl J Physiol Pharm Pharmacol. 2021; 11(6): 638-644. doi:10.5455/njppp.2021.11.12351202113042021


Web Style

Salami SA, Salahdeen HM, Idowu GO, Murtala BA, Alada ARA. Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. http://www.njppp.com/?mno=24657 [Access: October 02, 2021]. doi:10.5455/njppp.2021.11.12351202113042021


AMA (American Medical Association) Style

Salami SA, Salahdeen HM, Idowu GO, Murtala BA, Alada ARA. Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. Natl J Physiol Pharm Pharmacol. 2021; 11(6): 638-644. doi:10.5455/njppp.2021.11.12351202113042021



Vancouver/ICMJE Style

Salami SA, Salahdeen HM, Idowu GO, Murtala BA, Alada ARA. Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. Natl J Physiol Pharm Pharmacol. (2021), [cited October 02, 2021]; 11(6): 638-644. doi:10.5455/njppp.2021.11.12351202113042021



Harvard Style

Salami, S. A., Salahdeen, . H. M., Idowu, . G. O., Murtala, . B. A. & Alada, . A. R. A. (2021) Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. Natl J Physiol Pharm Pharmacol, 11 (6), 638-644. doi:10.5455/njppp.2021.11.12351202113042021



Turabian Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Gbolahan Olabisi Idowu, Babatunde Adekunle Murtala, and Abdul Rasak Akinola Alada. 2021. Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. National Journal of Physiology, Pharmacy and Pharmacology, 11 (6), 638-644. doi:10.5455/njppp.2021.11.12351202113042021



Chicago Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Gbolahan Olabisi Idowu, Babatunde Adekunle Murtala, and Abdul Rasak Akinola Alada. "Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger." National Journal of Physiology, Pharmacy and Pharmacology 11 (2021), 638-644. doi:10.5455/njppp.2021.11.12351202113042021



MLA (The Modern Language Association) Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Gbolahan Olabisi Idowu, Babatunde Adekunle Murtala, and Abdul Rasak Akinola Alada. "Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger." National Journal of Physiology, Pharmacy and Pharmacology 11.6 (2021), 638-644. Print. doi:10.5455/njppp.2021.11.12351202113042021



APA (American Psychological Association) Style

Salami, S. A., Salahdeen, . H. M., Idowu, . G. O., Murtala, . B. A. & Alada, . A. R. A. (2021) Tridax procumbens leaf extract elicits relaxation of rat mesenteric artery by activation of Na+ -K+ ATPase / Na+ - Ca2+ exchanger. National Journal of Physiology, Pharmacy and Pharmacology, 11 (6), 638-644. doi:10.5455/njppp.2021.11.12351202113042021