E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research

Online Publishing Date:
27 / 02 / 2016

 


Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Da'i.


Abstract
Background: Black rice bran contains bioactive compounds; previous studies have shown the ethanol extract of black rice bran (EEBRB) can decrease levels in diabetic rats.

Aims and Objective: To elucidate the mechanism of antidiabetic effect of EEBRB.

Materials and Methods: This research was carried out by setting a few parameters for antidiabetic. The effects of inhibition of the enzyme alpha-glucosidase performed with in vitro studies using the extracted enzyme of intestines of mice. A total of 5 mL alpha-glucosidase enzyme was incubated for 5 min with EEBRB 1 mL with a serial concentration in phosphate buffer pH 7.2. Enzyme inhibitory effect was determined by measuring the concentration of formed glucose with Lane–Eynon method. The insulin level and glucose-lowering activity were determined on rats induced by alloxan 150 mg/kg and treated with EEBRB. Treated rats for 28 days were sacrificed and were prepared for histopathological observation of pancreas.

Result: EEBRB was able to inhibit the activity of the enzyme a-glucosidase on glucose with IC50 values of 121 ± 12.3 mg%. The EEBRB extract dose of 100 mg/kg was able to decrease blood sugar levels to 151 ± 38.58 mg/dL. The level of insulin in diabetic rats administered by EEBRB dose of 50, 100, and 200 mg/kg was obtained 6.52 ± 5.94, 11.5 ± 3.5, and 15.20 ± 9.5 ng/mL, respectively. Meanwhile, histopathological observations showed an improvement on the pancreatic beta cells.

Conclusion: Antidiabetic mechanism of EEBRB is associated with increased insulin levels because of regeneration of pancreatic beta cells in diabetic rats.

Key words: Black Rice Bran; Antidiabetic; Insulin Level; Pancreatic Beta Cells


 
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How to Cite this Article
Pubmed Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590


Web Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. https://www.njppp.com/?mno=207825 [Access: March 14, 2024]. doi:10.5455/njppp.2015.5.1111201590


AMA (American Medical Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590



Vancouver/ICMJE Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. (2016), [cited March 14, 2024]; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590



Harvard Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai (2016) Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590



Turabian Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. 2016. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590



Chicago Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. "Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 106-110. doi:10.5455/njppp.2015.5.1111201590



MLA (The Modern Language Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. "Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 6.2 (2016), 106-110. Print. doi:10.5455/njppp.2015.5.1111201590



APA (American Psychological Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai (2016) Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590