E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


In vitro study of contractile responses of bowel with Hirschprung Disease

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay.

Abstract
Background: Hirschsprung’s disease (HD) is a congenital disorder in neonates which results from the absence of parasympathetic ganglion cells in the myenteric and submucosal plexus of the rectum, possibly extending to the colon.

Aims & Objective: To assess the contractile status of intestinal tissue of HD for better understanding of motility problems.

Materials and Methods: Circular muscle strips were prepared from freshly excised specimens of HD obtained from pediatric surgery operation theatre. The in vitro contractile activity of the tissue was assessed in an organ bath filled with physiological solution.

Results: Spontaneous contractions were recorded in 66% cases. Dose-dependent increase in response with the application of histamine and acetylcholine (0.1–100 μM) was observed. Responses of histamine were significantly (P < 0.05) blocked by pre-application of H1 blocker pheniramine (80%). Pre-application of atropine significantly (P < 0.05) blocked (62%) acetylcholine responses.

Conclusion: Large number of cases showed spontaneous contractions indicating functionally normal tissue.

Key words: Acetylch‾ Hirschprung disease; contractile responses; Histamine; Neonate


 
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How to Cite this Article
Pubmed Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. In vitro study of contractile responses of bowel with Hirschprung Disease. Natl J Physiol Pharm Pharmacol. 2015; 5(1): 10-13. doi:10.5455/njppp.2015.5.260620141


Web Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. In vitro study of contractile responses of bowel with Hirschprung Disease. http://www.njppp.com/?mno=161052 [Access: November 21, 2018]. doi:10.5455/njppp.2015.5.260620141


AMA (American Medical Association) Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. In vitro study of contractile responses of bowel with Hirschprung Disease. Natl J Physiol Pharm Pharmacol. 2015; 5(1): 10-13. doi:10.5455/njppp.2015.5.260620141



Vancouver/ICMJE Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. In vitro study of contractile responses of bowel with Hirschprung Disease. Natl J Physiol Pharm Pharmacol. (2015), [cited November 21, 2018]; 5(1): 10-13. doi:10.5455/njppp.2015.5.260620141



Harvard Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay (2015) In vitro study of contractile responses of bowel with Hirschprung Disease. Natl J Physiol Pharm Pharmacol, 5 (1), 10-13. doi:10.5455/njppp.2015.5.260620141



Turabian Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. 2015. In vitro study of contractile responses of bowel with Hirschprung Disease. National Journal of Physiology, Pharmacy and Pharmacology, 5 (1), 10-13. doi:10.5455/njppp.2015.5.260620141



Chicago Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. "In vitro study of contractile responses of bowel with Hirschprung Disease." National Journal of Physiology, Pharmacy and Pharmacology 5 (2015), 10-13. doi:10.5455/njppp.2015.5.260620141



MLA (The Modern Language Association) Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay. "In vitro study of contractile responses of bowel with Hirschprung Disease." National Journal of Physiology, Pharmacy and Pharmacology 5.1 (2015), 10-13. Print. doi:10.5455/njppp.2015.5.260620141



APA (American Psychological Association) Style

Shilpa Pandey, Maloy B Mandal, Ajay N Gangopadhyay (2015) In vitro study of contractile responses of bowel with Hirschprung Disease. National Journal of Physiology, Pharmacy and Pharmacology, 5 (1), 10-13. doi:10.5455/njppp.2015.5.260620141





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